Chlorophyll Fluorescence Imager

Chlorophyll Fluorescence Imager

I. Product Overview

The Chlorophyll Fluorescence Imaging System is a professional instrument for plant photosynthesis research. Utilizing a high-sensitivity CMOS camera and LED light source control, it enables rapid, non-destructive detection and imaging analysis of chlorophyll fluorescence in plant leaves. The system integrates two core functions: OJIP rapid fluorescence kinetics measurement and PAM modulated fluorescence measurement, enabling comprehensive evaluation of plant photosynthetic system activity, light use efficiency, and responses to environmental stress.

Featuring a modular design with highly integrated hardware and software, the system offers a user-friendly graphical interface supporting bilingual Chinese-English switching. It is widely applicable for research and teaching in plant physiology, ecology, agricultural science, environmental science, and related fields.

II. Technical Specifications

Camera:

Resolution: 1608(H) × 1104(V)

Pixel Size: 9µm × 9µm

★Frame Rate: 100 fps

Pixel Depth: 12 bit

Interface Type: USB 3.0

Lens and Optical Parameters

★Focal Length: 12mm

★Maximum Aperture: F/2.8 (dynamically adjustable)

Horizontal Field of View (HFOV): Approx. 62.11°

Vertical Field of View (VFOV): Approx. 44.83°

Maximum Imaging Range: 50cm × 35cm at standard working distance

Light Source Parameters

Blue Light Wavelength: 450 nm excitation source for fluorescence excitation

Red Light Wavelength: 630 nm photochemical light for PAM measurement

Far-Infrared Light: 730 nm for specific measurement requirements

LED Brightness Range: 1% - 100%, peak intensity up to 1440 µmol/(m²·s)

Measurement Range

1. OJIP Measurement

Measurement Duration: 0.1 - 1.0 seconds, adjustable, recommended 1 second

LED Brightness: 1% - 100%, corresponding to 90-1440 µmol/(m²·s), 5% increments

2. PAM Measurement

Dark Adaptation Time: 0 - 3600 seconds, can be set to 0 to skip

Light Cycle Count: 1 - 100 cycles, number of cycles during light adaptation phase

Monitoring Duration: 10 - 120 seconds/cycle, monitoring time per cycle

Recovery Time: 60 - 600 seconds, dark recovery monitoring duration

Photochemical Light Intensity: 1000 - 30000, red light 630nm LED brightness value

Saturation pulse (Fm): 15%–100%, dark adaptation saturation pulse (450nm blue light)

Saturation pulse (Fm'): 15%–100%, light adaptation saturation pulse (450nm blue light)

Sampling Interval: 0.1 - 10.0 seconds, data acquisition interval

Measurement Accuracy

1. Fluorescence Intensity Measurement

Dynamic Range: 12-bit (0-4095)

Signal-to-Noise Ratio: >100:1

Repeatability: CV < 3%

Linearity: R² > 0.999

Sensitivity: Capable of detecting faint fluorescence signals

2. Parameter Calculation Accuracy

Fv/Fm: ±0.005

ΦPSII: ±0.01

qP/qN: ±0.02

NPQ: ±0.1

PIABS: ±0.05

3. Time Resolution

OJIP Mode: Minimum acquisition interval 10 ms